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. 2015 Sep 23;10(9):e0138758. doi: 10.1371/journal.pone.0138758

Fig 6. ActinD1 expression level can be used as a marker of ontogenetic delay, which results in extended LS, but it is not a marker of biological age.

Fig 6

Differences in ActinD1 gene expression levels were determined between 28 days old diapausing females (O53-D), 15 days old (O53-15d) and control females (O53-C) reared for 28 days under non-diapause inducing conditions (12L:12D at 25°C), as well as between 28 days old non-diapausing females (CB05.08-ND), 20 days old (CB05.08-20d) and controls (CB05.08-C) reared for 28 days under non-diapause inducing conditions (12L:12D at 25°C). The reference gene RpL32 was used to normalize the expression values. Three biological replicates were used for each sample and the averages of the log-transformed values of 2-∆CT are presented with their respective S.E.M.. A two-tailed Student’s t test assuming equal variances was used to address if the averages of normalized ActinD1 expression levels of the different samples and treatments are significantly different (n.s. P > 0.05; * 0.05 > P > 0.01; ** 0.01 > P > 0.001; *** P < 0.001).