Fig 3. Real-time efflux of 1,2'-DNA in E. aerogenes strains.

Strains were grown in LB broth for 24 h at 37°C and cells were harvested by centrifugation. Cells were deenergized by an overnight incubation in Mg-PPB pH 7.0 with 5 μM CCCP and loaded with 1,2’-DNA. Cells were then washed, resuspended in Mg-PPB pH 7.0 and 1,2’-DNA efflux was monitored after rapid reenergization with glucose. RFU, relative fluorescence units. All values are means of at least three independent experiments.