Fig 1.

Colony PCR and Southern Blot confirming the deletion of glpX gene a) Colony PCR results of the potential double cross overs (DCOs): Lane 1: 1kb ladder, Lane 2: PCR amplification product of suicidal delivery vector FM152 is about 2kb, Lane 3: PCR product of one such potential DCO (Colony 11) is 2kb, Lane 4: PCR product of a WT Mtb is about 3kb, b) Southern blot: Lane 1: WT genomic DNA digest with BamH1 which gives a fragment of about 610 bp (lowermost band in lane 1 and 3), Lane 2: Genomic DNA digest of a potential DCO (Colony 3) with the 610 bp fragment missing, Lane 3: Same as Lane 1, Lane 4: Genomic DNA digest of a potential DCO (Colony 11) with the 600 bp fragment missing.