Table 1. Strains and plasmids used for the generation of Δglpx and glpx complement strains.
| Strains | Characteristics | Source |
|---|---|---|
| M. tuberculosis H37Rv | wild-type laboratory strain | ATCC 27294 |
| HG1 | M. tuberculosis ΔglpX | This study |
| HG2 | M. tuberculosis ΔglpX::pFM163 | This study |
| E.coli DH5α | Invitrogen | |
| p2NIL | manipulation vector | [18] |
| pGOAL19 | delivery cassette vector | [18] |
| pBluescript II SK+ | Stratagene | |
| pUC-Gm-int | [19] | |
| pFM143 | p2NIL::glpx | This study |
| pFM147 | pFM143::glpXΔ | This study |
| pFM152 | pFM147/with PacI cassette of pGoal19 | This study |
| pFM158 | pBluescript SK+::glpX (445/bp upstream) | This study |
| pFM163 | pFM158::intgm | This study |