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. 2015 Sep 23;10(9):e0138671. doi: 10.1371/journal.pone.0138671

Fig 1. Dense Fine Speckled nuclear pattern on HEp-2000® cells (titer 1:1280).

Fig 1

Indirect Immunofluorescence on Hep2000® cells with a fluorescein-conjugated secondary antibody (Immunoconcept) of the serum of a patient with anti-DFS70 antibodies. Picture was acquired using a LEICA/DM-LB2 microscope (x400 magnification) at 20°C, using camera DFC 300FX and acquisition software IM500 (Leica). Anti-DFS70 antibodies titer was determined by testing successive two-fold dilutions of the serum from 1/80 to 1/1280. Samples were classified as positive if well-defined immunofluorescence patterns were identified at 1/160 dilution.