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. Author manuscript; available in PMC: 2016 Oct 2.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Aug 25;465(4):739–745. doi: 10.1016/j.bbrc.2015.08.070

Figure 2. Beclin1 maintains persistent activity of NF-κB and Stat3 in HTLV-1-transformed T cells.

Figure 2

Whole cell lysates of NS-, Beclin1-, Atg5- or PI3KC3-depleted SLB-1 (A) and MT-2 (B) cells were examined with Western Blot (WB) using antibodies indicated in the figure. The activities of NF-κB, Stat3 and AP-1 in NS-, Beclin1-, Atg5- or PI3KC3-depleted SLB-1 (A) and MT2 (B) cells were measured by EMSA. IKKγ was silenced by the specific shRNA in SLB-1 (C) and MT-2 (D) cells, and the cell viability was determined accordingly. (E) The activities of NF-κB and Stat3 in Jurkat, MT1, MT2 and SLB-1 cells were analyzed with EMSA. (F) Whole cell lysates of NS-, Beclin1-, Atg5- or PI3KC3-depleted HEK293 cells were analyzed with immunoblot using the antibodies indicated in the figure. (G) NS-, Beclin1-, Atg5- or PI3KC3-depleted HEK293 cells were transfected with vector or Tax-HA. EMSA was applied to determine the activities of NF-κB and Stat3 (upper panel). The expression level of the Tax protein was shown in the lower panel as indicated in the figure.