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. Author manuscript; available in PMC: 2016 Aug 14.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Jun 23;464(1):221–228. doi: 10.1016/j.bbrc.2015.06.120

Figure 1. Niclosamide induces apoptosis of HTLV-1-transformed T cells.

Figure 1

A) MT-2 and MT-4 cells were treated with niclosamide. % of cell viability was determined by comparison of niclosamide vs solvent treated cells 48 hours after treatment using MTT assay. Experiments were performed in triplicate. *, p<0.05. **, p<0.01 as determined by 2-tail student t-test. B) and C) MT-2 cells (B) or MT-4 cells (C) were treated with niclosamide alone or with chloroquine.% of cell viability was determined after 72 hours using the method described in (A). D) and E) MT-4 cells were transduced with lentiviruses expressing NS (non-specific) shRNA, PI3KC3 shRNA (D) or Atg5 shRNA (E), and the cell viability was determined by trypan-blue exclusion assay. F) MT-2 and MT-4 cells were treated with niclosamide, and total protein lysates were analyzed with immunoblot to detect cleaved forms of caspase-3 (casp-3), -7, -9 and PARP 24 hours after niclosamide treatment.