Figure 4. Niclosamide inhibits multiple oncogenic signaling molecules.
A) Immunoblot of MT-2 and MT-4 cells treated with niclosamide. B) Total protein lysates from (A) were examined with anti-Bcl-2, -Bcl-xL and -Mcl-1 immunoblot. C) MT-2 and MT-4 cells were treated with niclosamideand the cellular protein extracts harvested at indicated time points were examined with immunoblot. D) Total protein lysates from (A) were examined with anti-p-ERK1/2 and anti-ERK1 immunoblot. E) Total protein lysates from (A) were examined with anit-Stat3 immunoblot. F) MT-4 cells were treated with 10 μM of niclosamide, and the nuclear extracts from indicated time points were examined with Stat3 probe EMSA. Oct-1 was used for control.