(A) Slices at the injection site (+6mm from bregma), and (B) slices 2 mm posterior to the injection site. Top: Raw images. Bottom: ROI of MnCl2 hyperintensity automatically thresholded at 195 on the grey scale (256 levels). To the right of the figure, corresponding slices of the Atlas of Yuasa et al, 2010. The following structures are hyperintense: primary motor cortex M1 (Brodmann area 4), the primary sensory cortex (3a), the cingulum (23–24), the premotor cortex (6c,6d), the parietal cortex (5), corpus callosum, corona radiata, caudate (Cd), putamen (Pu), internal (IGP) and external (EGP) globus pallidus, thalamic nuclei (VL: ventral lateral, RT: reticular), the internal capsule (ic). Note that MnCl2 follows the corpus callosum to the contralateral hemisphere most significantly with the highest doses.