Table 3. Quantification of CLas bacterial titers following qPCR from leaf petiole and midribs of the transgenic plants and controls grown under greenhouse conditions and exposed to free flying, potentially CLas positive psyllids.
The mean threshold cycle values (Ct) at specified time intervals are demonstrated.
| Transgenic line #2 | Transgenic line #4 | Transgenic line #9A | Transgenic line #11A | Transgenic line #18 | Control | |
|---|---|---|---|---|---|---|
| 6 months | - | - | - | 29.29± 2.3 | NT | 23.48±1 |
| 12 months | - | - | - | 23.63±1.4 | NT | 22.04±2.2 |
| 18 months | - | - | - | 21.19±3.1 | NT | 20.78±4.5 |
| 24 months | - | - | - | * | NT | * |
*; dead trees, NT; not tested, Standard errors were calculated from three replicates.