Fig. 7.

Histopathology assay of teratomas The teratoma formation of hiPSCs (left) by injection amount of 1 × 106 cells suspended in PBS into dorsal flanks of immunodeficient (SCID) mice and the tissue expansion from teratoma in vivo (right) by H&E staining and immunohistochemical staining (after three times repeated) using human E-cadherin (a-e: three germ layers including neural tissues (ectoderm), gut-like epithelial tissues (endoderm), striated muscle (mesoderm), cartilage (mesoderm), and keratin-containing epidermal tissues (ectoderm), respectively were observed. f-j: IHC staining demonstrates robust expression of human E-cadherin antibody on the surface of each germ layer of generated teratomas)