Table 2.
The primers sequence used for investigate the expression level of expression level of three germ layers genes of EBs by real-time PCR
| Primers name | Sequences | Germ layer | Products length (bp) | GenBank accession number |
|---|---|---|---|---|
| MSX1-F MSX1-R |
5′-AGCGCAGAGAAATCGGTGTC-3′ 5′-ATTCATCTTCCTGGCTGGC-3′ |
Mesoderm | 124 | NM_002448 |
| Brachyury T-F Brachyury T-R |
5′-CCTCGAATCCACATAGTGAGAG-3′ 5′-AAGAGCTGTGATCTCCTCGT-3′ |
117 | NM_001270484 | |
| PAX6-F PAX6-R |
5′-ACACACATTAACACACTTGAGC-3′ 5′-TGCTGTTGTTGCTTGAAGAC-3′ |
Ectoderm | 158 | NM_000280 |
| MAP2-F MAP2-R |
5′-AGGAAGGGAAGAGCATGTAAG-3′ 5′-ACACCACTTCCTCAACCACAG-3′ |
190 | NM_001039538 | |
| AFP-F AFP-R |
5′-AGGAGATGTGCTGGATTGTC-3′ 5′-ACATTGACCACGTTCCAGC-3′ |
Endoderm | 127 | NM_001134 |
| CK8-F CK8-R |
5′-ATGAGATCAATAAGCGTACAGAG-3′ 5′-TAGCTGCCTGAGGAAGTTGA-3′ |
140 | NM_001256282 |