Table 5.

The relationship of alkaline comet assay parameters between cultured hiPSCs on MEF, HDF and BD Matrigel matrix compare with normal healthy cells and H₂O₂ (positive control) analyzed by ANOVA test

Parameters	hiPSCs cultured on MEF feeder compare with positive control (H2O2 treatment)		hiPSCs cultured on MEF feeder compare with normal cells (healthy HDFs)
	Mean difference ± SE	p-value	Mean difference ± SE	p-value
Head area	471.599* ± 121.78	0.011	616.65 ± 58.234	0.7
Tail area	−4583.101* ± 190.087	0.000	687.36 ± 86.258	0.12
Head DNA%	64.372* ± 15.987	0.007	55.26 ± 7.36	0.000
Tail DNA%	−69.181* ± 0.85	0.000	54.66 ± 3.65	0.08
Tail length	−75.521* ± 2.464	0.000	112.3 ± 10.45	0.32
Comet length	−80.41* ± 2.856	0.000	92.41 ± 4.875	0.24
Tail moment	−86.43* ± 2.093	0.000	75.36 ± 24.23	0.000
	hiPSCs cultured on HDF feeder compare with positive control (H2O2 treatment)		hiPSCs cultured on HDF feeder compare with normal cells (healthy HDFs)
	Mean difference ± SE	p-value	Mean difference ± SE	p-value
Head Area	556.112* ± 98.236	0.02	487.25 ± 38.24	0.602
Tail Area	−6659.25* ± 150.3	0.000	587.89 ± 45.2	0.84
Head DNA%	78.36* ± 0.85	0.000	240 ± 58.3	0.000
Tail DNA%	−85.7* ± 0.722	0.032	64.36 ± 4.22	0.212
Tail Length	−84.38* ± 1.215	0.000	148 ± 24.684	0.000
Comet length	−100.2* ± 2.3	0.000	88.24 ± 4.98	0.264
Tail moment	−90.245* ± 1.66	0.000	105.7 ± 20.2	0.000
	hiPSCs cultured on BD Matrigel (feeder free) compare with positive control (H2O2 treatment)		hiPSCs cultured on BD Matrigel (feeder free) compare with normal cells (healthy HDFs)
	Mean difference ± SE	p-value	Mean difference ± SE	p-value
Head area	541.268* ± 109.122	0.022	652.8 ± 140.6	0.000
Tail area	−5212.25* ± 112.65	0.000	420.8 ± 78.2	0.405
Head DNA%	72.3* ± 0.854	0.000	452 ± 112.3	0.000
Tail DNA%	−84.9* ± 0.692	0.000	64.36 ± 10.2	0.000
Tail length	−92.24* ± 1.5	0.000	187.54 ± 24.684	0.000
Comet length	−87.134* ± 1.96	0.000	194.32 ± 14.54	0.2
Tail moment	−85.124* ± 1.44	0.000	144.5 ± 42	0.000

^*The mean difference is significant at p < 0.05

SE Std. Error