FIG 4.

Production of PRV-specific antibodies in immunized/challenged pigs. Groups of pigs (n = 5) were inoculated with different doses of rPRVTJ-delgE/gI-E2 or rPRVTJ-delgE or DMEM and challenged with the PRV TJ strain at the indicated time points. At 0, 3, and 6 days post-first immunization (dpi) and 0, 3, 6, 9, and 12 days postchallenge (dpc), serum samples were collected and tested for the presence of the anti-gB (A) and anti-gE (B) antibodies by using PRV antibody detection kits (Idexx, USA) according to the manufacturer's instructions. ELISA values are given in S/N (sample OD₆₅₀ value/negative OD₆₅₀ value) ratios. Samples with S/N ratios less than 0.6 were scored positive. Standard deviations were shown as error bars.