Fig. 6. The reduction of WST-8 shows the ethanol concentration dependent cytotoxicity. The degree of WST-8 reduction was determined by the CCK-8 Kit in primary glioblastoma cells (GBL-13, GBL-15) and glioblastoma cell lines (U87MG, U373MG). Four types of cells were treated with ethanol for 24 h, 48 h, and 72 h. Cell viability was determined by the CCK-8 Kit. Values are expressed as mean and SD. ^*p<0.05 compared with the control group.