Table 3.
Technique | G-CSF dose | Period of administration (d) | Stimulation regimen | Cell markers | Cryopreservant |
Administration of HSCs in blood | 10-20 μg/kg | 6-7 | 1 in 24 h | CD34+, CD45+ | 10%-20% DMSO |
HLA DR+, CD38+ | |||||
Gp130± | |||||
Administration of HSC and HPs in CSF | 5 μg/kg; double dose at day 4 | 5 | 2 in 24 h | CD34+, CD45- | 5%-10% DMSO + |
HLA DR±, CD38± | polyglucin | ||||
Gp130+ |
HSCs: Hematopoietic stem cells; HPs: Hematopoietic precursors; G-CSF: Granulocyte-colony stimulating factor.