Figure 8.

In vitro determination of nanofiber formation of RADA16-I and its ability to form nanofiber clots with red blood cells (RBCs). The premise of this assay is represented schematically where the presence of nanofiber-forming RADA16-I can interact with RBCs to maintain their suspension in solution (A), whereas their absence or inability for nanofiber formation results in RBC settling to the bottom (B). Standard dilutions of various controls reveal there is a necessary concentration of RADA16-I for this effect, the polymers used during film assembly (i.e., dextran sulfate and hyaluronic acid) are not inhibitory, and these polymers alone are not capable of forming these nanofiber clots (C). The RADA16-I eluted from these films remains capable of generating these nanofiber clots with RBCs (D).