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. 2015 Aug 17;7(10):835–841. doi: 10.1038/nchem.2325

Figure 4. Characterization of error-free operations of the entire AIRS system by gel electrophoresis.

Figure 4

a, Analysis of antibody mimicry P* generation by agarose gel (1.5%). L, 100-base-pair ladder. The image was taken under an ethidium bromide (EB) imaging channel (λexcitation = 520 nm, λemission = 605 nm). b, Validation of the AIRS analogue system performance with pathogen strand P0 by agarose gel (1%). M is the mixture of original present components in the AIRS. Here M = mixture of 200 nM AM, 200 nM BM, 200 nM PG, 50 nM CT, 0.5 U µl–1 Phi29 and 250 µM dNTP; incubation time, one hour. Lane 1, M + 0 nM FAM-labelled P0; lane 2, M + 100 nM FAM-labelled P0; lane 3, M + 500 nM FAM-labelled P0; lane 4, M + 100 nM FAM-labelled P0 + 2 U µl–1 SspI; lane 5, M + 500 nM FAM-labelled P0 + 2 U µl–1 SspI; L, 1 kb ladder. c, Validation of the AIRS analogue system performance with the pathogen strand P0-SARS by agarose gel (1%). M = mixture of 200 nM AM, 200 nM BM, 200 nM PG, 50 nM CT-SARS, 0.5 U µl–1 Phi29 and 250 µM dNTP; incubation time, one hour. Lane 1, M + 0 nM FAM-labelled P0-SARS; lane 2, M + 100 nM FAM-labelled P0-SARS; lane 3, M + 500 nM FAM-labelled P0-SARS; lane 4, M + 100 nM FAM-labelled P0-SARS + 2 U µl–1 SspI; lane 5, M + 500 nM FAM-labelled P0-SARS + 2 U µl–1 SspI; L, 1 kb ladder.