Figure 5. Acute in vivo effect of 1 in mice in an OGTT.

(a,c) Mice (WT and Ide−/− C57BL/6J) were treated with 1 at 50 mg kg⁻¹ (blue line) or with vehicle (grey line) intraperitoneally. Plasma glucose concentrations measured immediately before inhibitor injection, corresponding to t=−15 min, immediately before oral glucose challenge (3 g kg⁻¹, at t=0), and up to 180 min after it. Inserts represent AUC _{(−15 to 180)}. (b,d) : Insulin concentration measured in the same animals (WT and Ide−/− C57BL/6J) (1: blue bars; vehicle: grey bars) was measured at the indicated time points. One out of three independent experiments is shown. The experiment shown was performed as ‘cross-over', such that the group initially treated with vehicle was treated two days later with 1 and vice versa. Data are means±s.e.m (n=6 mice per group). two-sided t-test *P< 0.05; **P<0.01 ; ***P<0.001.