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. 2015 Feb 23;5(1):e1006089. doi: 10.1080/21565562.2015.1006089

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© 2015 The Author(s). © 2015 Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — Individualization is not sensitive to diet. Males reared on normal food and shifted within 3 d of eclosion to glucose food (light bars) for either 4 d (A) or 8 d (B) show no individualization defects compared to age matched siblings kept on normal food (dark bars). N ≥ 10 testes each condition. (C) oys^Δ⁶⁷ nes^Δ⁵² double mutant males collected within 5 d of eclosion and cultured on glucose food for 5 d show highly abnormal individualization, with an average of fewer than one normal IC per testis. Controls are balancer siblings cultured in parallel. N ≥ 17 testes each condition.