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. 2015 Feb 23;5(1):e1006089. doi: 10.1080/21565562.2015.1006089

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© 2015 The Author(s). © 2015 Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 6. — Drosophila PLA₂ homologs are expressed in the male reproductive tract. (A) Phylogenetic tree of PLA₂ family members from Saccharomyces cerevisiae (Sc), Drosophila melanogaster (Dm), and Homo sapiens (Hs). Tree was compiled using software provided by Interactive Tree of Life (itol.embl.de)⁸² and following classifications in ref. 24 (B) RT-PCR on cDNA (“c”) prepared from w¹¹¹⁸ reproductive tracts showing expression of CG6718, sws, CG10133, CG14507, CG17035, CG1583 and CG11124 (asterisks). Control PCR was run for each set of primers on genomic DNA (“g”). Actin-5c PCR was used as a control for cDNA integrity.