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. 2015 Sep 24;46(1):103. doi: 10.1186/s13567-015-0238-4

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© Kumar et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Detection methods for Yersinia ruckeri using loop-mediated isothermal amplification method. A: Agarose gel showing ERM-LAMP products of Y. ruckeri; Lane mar: 100 bp DNA ladder, lane Y. ruc: amplified Y. ruckeri LAMP product, lane Y. ruc dig: Hph I digested Y. ruckeri LAMP products of 87 and 108 bp, and lane veco: negative control. B: Visual detection of ERM LAMP products using SYBR Green I stain 1: 1: Negative control reaction using Rox- labelled probe, there is neither pellet nor red fluorescence, 2: positive control reaction using Rox- labelled probe, the pellet emitted red fluorescence; 3: positive sample by using FDR, emitted strong green fluorescence when exposed to UV light; 4: negative sample by using FDR, did not emit strong green fluorescence under UV light; 5: positive sample with green color using SYBR green I stain; 6: negative sample with orange color using SYBR green I stain (Image from Saleh et al. [35] with permission).