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. 2015 Sep 24;12:81. doi: 10.1186/s12977-015-0207-z

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© Stieh et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Native-PAGE determination of the Env conformations present after virus depletion. a Purified HIV-1_BaL virions were depleted by the antibody specified and separated on native-PAGE gels and western blots were probed with a gp41 (2F5, 4B3, 3D6) or gp120 (b12, 2G12, b6) antibody cocktail. The abundance of Env species were quantified relative to undepleted virus for blots probed with (b) gp120 antibodies or (c) gp41 antibodies. Blot is representative of at least 3 independent depletion experiments and quantification is the average of 3 images