Table 2.
Bacterial strains and plasmids.
| Strains/Plasmids | Relevant Characteristics | Reference or Source |
|---|---|---|
| B. japonicum | ||
| 61A101C | Wild type, Cmr | Nitragin (Milwaukee, WI, USA) |
| JS015 | Strain 61A101C waaL::Km, Kmr | This study |
| CS015 | JS015/pBBRL, Smr, Kmr | This study |
| E. coli | ||
| DH5α | supE44, ΔlacU169 (Φ80 lacZΔM15), hsdR17, recA1, endA1, gyrA96, thi-1, relA1 | Invitrogen (Carlsbad, CA, USA) |
| S17-1 | recA pro (RP4-2Tet::Mu Kan::Tn7) | [39] |
| Plasmids | ||
| pGEM-T easy | PCR cloning vector, Ampr | Promega (Madison, WI, USA) |
| p34s-Km | Vector containing Kmr cassette, Kmr | [40] |
| pTRL | pGEM T easy vector containing 1.5 kb fragment of waaL gene, Ampr | - |
| pTRLK | pTRL derivative waaL gene disruption by deletion of BssHII fragment and insertion of Kmr cassette, Kmr | - |
| pJQ200SK | Suicide vector, sacB, Gmr | [41] |
| pJQRLK | pJQ200SK-waaL::Km, Gmr, Kmr | This study |
| pIJ778 | pBluescript KS(+) derivative containing the streptomycin/spectinomycin resistance gene aadA, Smr | [42] |
| pBBR1MCS-2 | Broad host range expression vector, mod, rep, lacZ, Kmr | [43] |
| pBBRL | pBBR1MCS-2 carrying the 1.5 kb fragment of waaL gene, Kmr | This study |
| pBBRL-Sm | pBBRL derivative containing the Smr gene, Kmr gene disruption by deletion of NcoI fragment, Smr | This study |