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The effects of the mutation in czcS1 and czcS2 on the transcription of czcR1and czcR2. Transcription of czcR1 and czcR2 was analyzed by qRT-PCR in the wild-type X4 strain and mutant strains grown with 300 μM Zn2+ or Cd2+. The amount of mRNA is represented relative to the wild-type X4 strain cultivated in the same concentration of metal ions. Error bars represent the standard deviations of three determinations.
