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. 2015 Jul 27;16(8):17018–17028. doi: 10.3390/ijms160817018

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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CCND2 siRNA transfection inhibited HaCaT cell proliferation by blocking cell cycle at G1 phase. (A) CCND2 siRNA transfection led to a significant reduction of mRNA expression of CCND2 in HaCaT cells both at 24 h (73.79% ± 11.45%) and 48 h (51.18% ± 8.85%); (B) Cell viability analysis showed that CCND2 siRNA transfection inhibited HaCaT cell proliferation at 24 h (67.98% ± 7.31%) and 48 h (67.45% ± 6.70%); (C) FCM analysis showed the effect of CCND2 siRNA transfection on cell cycle progression, and G1 phase arrest was obvious at 24 h (55.51% ± 6.18%) and 48 h (70.19% ± 4.10%) compared with negative control (42.98% ± 2.48%). (NC, negative control. * Compared with NC, p < 0.05).

CCND2 siRNA transfection inhibited HaCaT cell proliferation by blocking cell cycle at G1 phase. (A) CCND2 siRNA transfection led to a significant reduction of mRNA expression of CCND2 in HaCaT cells both at 24 h (73.79% ± 11.45%) and 48 h (51.18% ± 8.85%); (B) Cell viability analysis showed that CCND2 siRNA transfection inhibited HaCaT cell proliferation at 24 h (67.98% ± 7.31%) and 48 h (67.45% ± 6.70%); (C) FCM analysis showed the effect of CCND2 siRNA transfection on cell cycle progression, and G1 phase arrest was obvious at 24 h (55.51% ± 6.18%) and 48 h (70.19% ± 4.10%) compared with negative control (42.98% ± 2.48%). (NC, negative control. * Compared with NC, p < 0.05).