Table 1.
Nucleotide sequences, amplification sites, GenBank accession numbers, amplimer sizes and PCR reaction conditions for each primer pair are shown.
| Gene | Primer | Nucleotide Sequence (5′→3′) | GenBank ID | Annealing Temperature | Amplimer Size (bp) |
|---|---|---|---|---|---|
| Collagen II (Col2a1) | sense | GGA CCC AAA GGA CAG ACG G (1191–1210) | NM_204426 | 59 °C | 401 |
| antisense | TCG CCA GGA GCA CCA GTT (1573–1591) | ||||
| Collagen IX (Col9a1) | sense | GGG ACAA GAG GAA TAA ACG (1732–1750) | NM_001100911.1 | 52 °C | 163 |
| antisense | CTG GTA AAC CTG GCA ATC (1877–1894) | ||||
| Collagen X (Colxa1) | sense | TCT GGG ATG CCG CTT GTC (1681–1698) | NM_009925.4 | 56 °C | 261 |
| antisense | CGT AGG CGT GCC GTT CTT (1924–1941) | ||||
| PACAP (ADCYAP1) | sense | CTT CGC ACT ACG AGC AGG (156–163) | NM_001001291 | 52.5 °C | 198 |
| antisense | TTG ACA GCC ATT TGT TTC C (335–363) | ||||
| PAC1 (ADCYAP1R1) | sense | GTC AGA CAA CCA GGA TTA C (435–453) | NM_001098606 | 49 °C | 141 |
| antisense | TGG ATA AAG TTC CGA GTG (559–575) | ||||
| VPAC1 (VIPR1) | sense | GTT CTA TGG CAC GGT CAA (376–393) | NM_001097523 | 52 °C | 216 |
| antisense | AGC AAT GTT CGG GTT CTC (573–590) | ||||
| VPAC2 (VIPR2) | sense | TCG GAA CTA CAT CCA TCT (477–497) | NM_001014970 | 48 °C | 177 |
| antisense | TTT GCC ATA ACA CCA TAC (636–653) | ||||
| SHH (Shh) | sense | TCA GTG GCA GCG AAA TCA (787–804) | NM_204821.1 | 56 °C | 168 |
| antisense | CAT CCG GTC GAG GAA GGT (937–954) | ||||
| IHH (Ihh) | sense | TCG CCT ACA AGC AGT TCA GCC (455–475) | NM_204957.1 | 60 °C | 155 |
| antisense | GCC GGT GTT CTC CTC GTC CT (590–609) | ||||
| PTHrP (Pthlh) | sense | TAC GGA AGA TCA GTA GAG G (155–173) | NM_001174106.1 | 46 °C | 191 |
| antisense | GTA GCA GGC TTA GGG TTA (328–345) | ||||
| Smoothened (SMO) | sense | TCT GCT TCG TGG GTT ACA AG (843–862) | XM_414970.4 | 56 °C | 350 |
| antisense | TGG GAT GGG TTT ATT GGT CT (1173–1192) | ||||
| Gli1 (Gli1) | sense | CTC ACC CAC CCA GCA TCA G (2604–2622) | XM_004950861.1 | 58 °C | 244 |
| antisense | AAT CCC TCC TCC ATC TCC CT (2828–2847) | ||||
| Gli2 (Gli2) | sense | TTG CTC CAA GGC TTA CTC (1553–1570) | M_001271901.1 | 50 °C | 174 |
| antisense | TTA CAG ACA TAG GGT TTC TCA T (1705–1726) | ||||
| Gli3 (Gli3) | sense | TCA CCC GTA CAT TAA CCC (579–596) | NM_001271903.1 | 52 °C | 270 |
| antisense | CTT GGA CTC GGA AAC CTG (831–848) | ||||
| GAPDH (GAPDH) | sense | GAG AAC GGG AAA CTT GTC AT (238–258) | NM_204305 | 54 °C | 556 |
| antisense | GGC AGG TCA GGT CAA CAA (775–793) |