Table 2.
Tables of antibodies used in the experiments.
| Antibody | Host Animal | Dilution | Distributor and Cat No. |
|---|---|---|---|
| Anti-PAC1 | rabbit, polyclonal, | 1:600 | Sigma-Aldrich, St. Louis, MO, USA; P8872 |
| Anti-VPAC1 | rabbit, polyclonal, | 1:800 | Alomone Labs., Jerusalem, Israel; AVR-001 |
| Anti-VPAC2 | rabbit, polyclonal, | 1:600 | Abcam, Camridge, UK; ab28624 |
| Anti-Coll. II. | rabbit, polyclonal, | 1:100 | Novus Biologicals, Littleton, CO, USA; NB600-844 |
| Anti-Coll. IX. | rabbit, polyclonal, | 1:800 | Abcam, Camridge, UK; ab134568 |
| Anti-Coll. X. | rabbit, polyclonal, | 1:800 | Sigma-Aldrich, St. Louis, MO, USA; C7974 |
| Anti-SHH | rabbit, polyclonal, | 1:600 | Cell Signaling, Danvers, MA, USA; C9C5 |
| Anti-IHH | rabbit, polyclonal, | 1:600 | Millipore, Billerica, MA, USA; MABF23 |
| Anti-PTHrP | mouse, monoclonal, #677939 | 1:300 | R&D Systems, Minneapolis, MN, USA; MAB6734 |
| Anti-Smoothened | mouse, monoclonal, #2D10 | 1:500 | Sigma-Aldrich, St. Louis, MO, USA; SAB1412475 |
| Anti-Gli1 | rabbit, polyclonal, | 1:600 | Cell Signaling, Danvers, MA, USA; V812 |
| Anti-Gli2 | rabbit, polyclonal, | 1:500 | Sigma-Aldrich, St. Louis, MO, USA; SAB2900411 |
| Anti-Gli3 | rabbit, polyclonal, | 1:500 | Sigma-Aldrich, St. Louis, MO, USA; HAP005534 |
| Anti-Actin | mouse, monoclonal, #AC-15 | 1:10,000 | Sigma-Aldrich, St. Louis, MO, USA; A5441 |