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. 2015 Jul 30;16(8):17422–17444. doi: 10.3390/ijms160817422

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of photoproducts excision activity after UVC irradiation. Mock and APP751-expressing cells were cultured for 48 h and either left untreated or UVC-irradiated. Total cell extracts were generated, and photoproducts excision capacity was analyzed on damaged genomic DNA substrates for 30 min using a modified version of the comet assay. The excision capacity of cell extracts was analyzed as the mean tail intensity of the comet. Three biological replicates were tested in triplicate in three independent experiments. The mean tail intensity of each cell extract (n = 3) was calculated and then a Student’s t-test was performed. Ctrl-corresponds to reaction buffer alone. T4: T4-endonuclease, positive control for the excision of the UV-induced DNA damage; *^#§ data significantly different from ctrl-, *** (p = 0.0005); ^### (p = 0.0005); ^§§§ (p = 0.0005).