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. 2015 Aug 6;16(8):18293–18311. doi: 10.3390/ijms160818293

Figure 5.

Figure 5

MTX administration increased mRNA expression of receptor activator of NF-κB ligand (RANKL), osteoprotogerin (OPG), interleukin-6 (IL-6), and IL-1β in the metaphysis bone compared to control rats. (A,B) Quantitative RT-PCR of osteoclastogenesis regulators RANKL and OPG, respectively, within the metaphysis; (C) Ratio of relative mRNA expression of RANKL to OPG; (D–F) Quantitative RT-PCR of pro-inflammatory cytokines IL-6, IL-1β, and tumor necrosis factor-alpha (TNF-α), respectively. n = 6, * p < 0.05 and ** p < 0.01 vs. control; # p < 0.01 between Gen and MTX alone groups, and δ p < 0.05 between Gen alone and Gen/MTX groups.