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. 2015 Aug 7;16(8):18384–18395. doi: 10.3390/ijms160818384

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Effect of co-treatment with hesperidin and MG132 (protease inhibitor) on (a) melanin contents and (b) MITF protein expressions in B16F10 cells. Cells were incubated with MG132 with or without hesperidin. Melanogenesis-related protein expressions were analyzed by Western blot. Each percentage value for the treated cells was reported relative to that in α-MSH-stimulated cells. Data were expressed as mean ± SD of three independent experiments carried out in triplicates. ** p < 0.01 as compared to the one treated with hesperidin (30 μM) only.