Table 3.

Key commonly altered metabolites detected by GC–MS for Hu-H1 and Strain V.

Hu-H1/Strain V	Metabolite	RT (min)	MZ	VIP-Value (OPLS-DA)		p-Value (t-test) Hu-H1 Strain V		Fold Change Hu-H1/CON Strain V/CON
				Hu-H1	Strain V
1	Citric acid	14.63	273	2.42	1.66	1.10 × 10−10	4.82 × 10−16	−1.49	−3.22
2	l-CysteineL	11.61	218	1.86	1.44	0.000129	1.89 × 10−7	−1.34	−3.31
3	Pyruvic acid	5.05	174	2.19	1.09	4.58 × 10−7	4.94 × 10−5	−1.29	−2.4
4	l-Aspartic acid	11.2	233	2.14	1.52	0.00000144	1.61 × 10−8	−1.18	−2.06
5	Desmosterol	25.47	343	1.94	1.5	0.0000461	1.60 × 10−8	−1.16	−2.84
6	Margaric acid	17.6	117	2.35	1.42	3.73 × 10−9	4.04 × 10−7	−1.03	−0.97
7	Glycerol-3-phosphate	14.08	357	1.61	1.27	0.00167	3.36 × 10−5	−0.8	−1.28
8	l-Alanine	5.69	116	1.95	1.51	0.0000402	1.14 × 10−8	−0.67	−1.56
9	Myo-Inositol	17.38	305	1.87	1.41	0.000112	6.71 × 10−7	−0.66	−1.2
10	Serine	9.18	204	2.03	1.59	0.0000118	4.36 × 10−11	−0.58	−1.7
11	Phenylalanine	12.43	218	1.16	1.38	0.0334	1.82 × 10−6	−0.56	−2.37
12	l-Threonine	9.54	218	1.84	1.55	0.000159	6.11 × 10−10	−0.53	−1.41
13	Pyroglutamic acid	11.19	156	1.27	1.15	0.018	3.22 × 10−4	−0.49	−0.91
14	Malic acid	10.82	233	1.31	1.38	1.46 × 10−2	1.44 × 10−6	−0.47	−1.64
15	l-Methionine	11.15	176	1.23	1.42	2.31 × 10−2	3.76 × 10−7	−0.46	−1.97
16	l-Proline	8.29	142	1.15	1.4	3.45 × 10−2	9.06 × 10−7	−0.31	−1.22
17	l-Valine	7.21	144	1.41	1.51	7.71 × 10−3	7.91 × 10−9	−0.24	−0.95
18	l-Leucine	7.97	158	1.24	1.46	2.22 × 10−2	9.99 × 10−8	−0.21	−0.91
19	l-Isoleucine	8.27	158	1.16	1.45	3.36 × 10−2	1.34 × 10−7	−0.2	−0.78
20	Eicosanoic acid	20.1	117	1.17	1.02	3.19 × 10−2	1.95 × 10−3	0.27	0.45
21	Ethanolamine	7.9	174	1.35	1	1.13 × 10−2	2.52 × 10−3	0.77	0.77
22	Methylsuccinic acid	8.69	261	1.86	1.25	1.42 × 10−4	0.0000518	1.79	1.64