Table 4.

Key differential metabolites detected by GC–MS for Hu-H1/Strain V.

Hu-H1/Strain V	Metabolite	RT (min)	MZ	VIP-Value	p-Value (t-test)	Fold Change *
				(OPLS-DA)
1	Glycolic acid	5.35	177	1.36	3.71 × 10−5	−0.75
2	Myo-Inositol-1-phosphate	20.26	318	1.06	8.76 × 10−4	−0.68
3	d-Fructose-6-phosphate	19.4	315	1.6	4.89 × 10−5	−0.62
4	Glycerol	8.05	205	1.11	1.86 × 10−5	−0.37
5	Cholesterol	25.21	129	1.05	2.42 × 10−2	−0.36
6	Desmosterol	25.47	343	1.6	3.96 × 10−2	−0.34
7	Lanosterol	25.35	458	1.26	7.60 × 10−2	−0.25
8	Citric acid	14.63	273	1.53	1.62 × 10−1	−0.2
9	d-Glucose-6-phosphate	19.5	387	1.59	7.22 × 10−2	0.39
10	Myo-Inositol	17.38	305	1.09	4.37 × 10−2	0.48
11	l-Isoleucine	8.27	158	1.41	1.43 × 10−5	0.58
12	Ribulose-5-phosphate	17.72	357	1.2	1.52 × 10−3	0.61
13	l-Leucine	7.97	158	1.41	1.25 × 10−5	0.7
14	l-Valine	7.21	144	1.53	5.61 × 10−7	0.71
15	Glycine	8.43	174	1.5	1.58 × 10−6	0.83
16	d-Fructose	15.28	103	1.55	1.08 × 10−3	0.88
17	l-Threonine	9.54	218	1.39	2.11 × 10−5	0.89
18	l-Alanine	5.69	116	1.43	8.28 × 10−6	0.89
19	l-Proline	8.29	142	1.37	3.67 × 10−5	0.92
20	Serine	9.18	204	1.49	1.66 × 10−6	1.13
21	d-Glucose	15.43	205	1.51	3.58 × 10−8	1.14

* Fold change was calculated as the logarithm of the average mass response (area) ratio between the two classes (i.e., fold change = log2(Hu-H1/Con)). Thus, positive fold-change values indicate significantly higher levels in Hu-H1 relative to Con, and negative fold change values indicate significantly lower levels in Hu-H1 relative to Con.