Skip to main content
. 2015 Aug 21;16(8):19920–19935. doi: 10.3390/ijms160819920

Figure 1.

Figure 1

The results of phage display selection against Hsp90α. (A) Monoclonal ELISA for 64 scFv clones selected after the third round of panning against Hsp90α. Bacterial supernatants containing soluble antibody fragments were applied onto the target protein immobilized directly on the plastic surface of 96-well Nunc MaxiSorp® plate. The binding clones were detected with the use of monoclonal mouse antibody 9E10. Of all clones, 51 showing the highest absorption at 450 nm were selected for further analysis by SPR on Biacore® 3000. The best clone scFv47 is highlighted with orange; (B,C) SPR response curves for measurements of scFv47 affinity for Hsp90. Pure scFv47 in five different concentrations was injected on Hsp90 isoform α (A) or β (B) immobilized on CM5 sensor chip. The calculations of kinetic constants were done with the use of BIAevaluation 4.1 software.