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. 2015 Aug 21;16(8):19920–19935. doi: 10.3390/ijms160819920

Figure 2.

Figure 2

The results of affinity maturation for scFv47 clone. (A) Monoclonal ELISA for scFv clones selected after the second round of panning against Hsp90α. Of all clones, 35 were selected for further analysis by SPR on Biacore® 3000. The best clone scFvA4 is highlighted with orange; (B,C) Binding curves for scFvA4 clone against Hsp90α and Hsp90β, respectively. Varied concentrations of purified protein were injected on two CM5 sensor chips immobilized with Hsp90 isoforms. The BIAevaluation software was used for calculations of kinetic constants; (D) The amino acids selected at CDR1, CDR2 and CDR3 of parental and affinity matured clones.