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The immunofluorescence detection of intracellular Hsp90 in fixed and permeabilized. (A) MDA MB 453 cell line; and (B) MDA MB 231 cell line. The intracellular Hsp90 was stained green either by scFv47-FITC, scFvA4-FITC or anti-Hsp90/secondary goat anti-mouse Alexa 488-conjugated antibodies. Nuclei were stained with DAPI (blue) and the cytoskeletal actin filament network was visualized with Alexa 594 phalloidin (red). Original magnification ×20, for all panels.
