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Calreticulin (CRT) exposure detected by immunofluoroscence assay after treatment of X12-PG. (A–D) were respectively treated with 0, 400, 800, 1600 μg/mL X12-PG for 48 h, then incubated with primary antibody (Anti-Calreticulin, diluted 1:200 in blocking buffer) and detected with the FITC-labeled secondary antibody. Fluorescence intensity was visualized by immunofluorescence microscopy (×200).
