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. 2015 Sep 24;14:152. doi: 10.1186/s12934-015-0337-y

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© Zhang et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Gene replacement in S. avermitilis NA-108. a Schematic description of the gene replacement of aveDH2-KR2 in S. avermitilis NA-108 with milDH2-ER2-KR2 from S. bingchenggensis via double crossover. b PCR analysis with genomic DNA from S. avermitilis NA-108, S. avermitlis AVE-T27 and S. bingchenggensis, using primers E1 and E2. Lane 1 S. bingchenggensis; Lane 2 S. avermitilis AVE-T27; Lane 3 S. avermitilis NA-108; Lane 4 DNA ladder. c Schematic description of the gene replacement of aveLAT-ACP in S. avermitilis AVE-T27 with milLAT-ACP from S. bingchenggensis via double crossover. d PCR analysis with genomic DNA from S. avermitilis AVE-T27, S. avermitlis AVE-H39 and S. bingchenggensis, using primers V1 and V2. Lane 1 DNA ladder; Lane 2 S. bingchenggensis; Lane 3 S. avermitilis AVE-T27; Lane 4 S. avermitilis AVE-H39