Table 2.
Strains and plasmids used in this study
| Strains/plasmids | Relevant characteristicsa | References |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | Host strain for cloning | Invitrogen |
| ET12567/pUZ8002 | Donor strain for conjugation | Kieser et al. [37] |
| S. bingchenggensis | The milbemycin-producing strain | Wang et al. [14] |
| S. avermitilis | ||
| NA-108 | Mutant strain of S. avermitilis NEAU1069 producing “B” components of avermectins as main secondary metabolites | This study |
| AVE-T27 | Mutant strain of NA-108 with the replacement of aveDH2-KR2 by milDH2-ER2-KR2 | This study |
| AVE-H39 | Mutant strain of AVE-T27 with the replacement of aveLAT-ACP by milLAT-ACP | This study |
| Plasmids | ||
| pUC19 | Cloning vector for E. coli, Amp R | TaKaRa |
| pKC1139 | E. coli-Streptomyces shuttle vector, Am R | Kieser et al. [37] |
| pER-1 | pUC19 derivative containing aveDH2-KR2 upstream fragment, Amp R | This study |
| pER-2 | pUC19 derivative containing aveDH2-KR2 upstream and downstream fragments, Amp R | This study |
| pER-3 | pUC19 derivative containing milDH2-ER2-KR2 together with aveDH2-KR2 upstream and downstream fragments, Amp R | This study |
| pER-4 | pKC1139 derivative containing milDH2-ER2-KR2 together with aveDH2-KR2 upstream and downstream fragments, Am R | This study |
| pLM-1 | pMD19-T derivative containing milLAT-ACP together with aveLAT-ACP upstream and downstream fragments, Amp R | This study |
| pLM-2 | pKC1139 derivative containing milLAT-ACP together with aveLAT-ACP upstream and downstream fragments, Amp R | This study |
a Amp R ampicillin resistance, Am R apramycin resistance