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CSA increases Treg percentages in a CD44 dependent manner. Fold increase (FI) in the percentage of GFP/Foxp3+ cells of (a) CD44-containing or (b) CD44-deficient, murine CD4+ T cells after 3 days of culture in the settings of anti-CD3 and anti-CD28 with or without CSA in various concentrations. (c) Fold increase (FI) in percentage of GFP/Foxp3+ cells after 3 days of culture in the setting of anti-CD3 and anti-CD28 alone or in conjunction with anti-CD44 Ab. n = 5 replicate wells.
