Fig 5. Fractionation of cattle saliva proteins.

(a) SDS-PAGE analysis of fractionated cattle saliva proteins. Proteins present in the cattle saliva were fractionated by gel filtration chromatography. An aliquot of each fraction was used for SDS-PAGE analysis, following which the acrylamide gel was stained with silver stain. Collected fractions were then divided as indicated (based on molecular weight distribution of proteins) into five sample groups (called here as sample A, B, C, D and E) for further use. (b) Enhancement effect of fractionated cattle saliva. Cellulase-catalyzed cellulose degradation assay (in triplicate) was performed using one of the fractionated saliva samples (A, B, C, D, or E) or Mix sample as an additive, and the amount of reducing sugar produced in each case was measured. Error bars indicate ± standard deviations. Values labeled with asterisk are statistically different as established by Student's t-test (P < 0.05).