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. 2015 Sep 24;9(9):e0004086. doi: 10.1371/journal.pntd.0004086

Fig 6. Kaplan-Meier survival curves for adult S. mansoni worms treated in vitro with PZQ+OMP, PZQ or OMP.

Fig 6

Adult worm couples were treated with a synergistic combination of PZQ+OMP (blue line), with PZQ alone (red line) or with OMP alone (black line). The assays were performed with paired adult worm couples (20 couples for each of the three conditions in each of the two biological replicates) and the fraction of surviving worms was recorded after 2, 24, 48, 72, 96 and 120 hours; the Kaplan-Meier survival curve was calculated using all the events of the two biological replicas together, and the Log-rank Mantel-Cox statistical test was used to calculate the significance of the Hazard Ratio between OMP+PZQ and PZQ alone. The effect of the synergistic combination was analyzed for each gender separately: (A) for scoring males percent survival, couples were incubated in the presence of 150 ng/ml PZQ, with or without 25 μg/ml OMP, or in the presence of 25 μg/ml OMP alone; and (B) for scoring females percent survival, couples were incubated in the presence of 532 ng/ml PZQ, with or without 25 μg/ml OMP, or in the presence of 25 μg/ml OMP alone.