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. 2015 Sep 24;11(9):e1005513. doi: 10.1371/journal.pgen.1005513

Fig 3. Altered ETHR expression in central ensembles modifies scheduling of the ecdysis FAP.

Fig 3

(A) Knockdown of ETHR expression using three independent ETHR-RNAi lines: UAS-ETHR-IR1, UAS-ETHR-IR2, and UAS-ETHR-sym carrying UAS-Dicer2. ETHR knockdown in kinin neurons reduced pre-ecdysis duration. ETHR knockdown in CAMB neurons (Pburs-Gal4) delays the switch to ecdysis behavior. A model below depicts how ETHR knockdown in kinin neurons or CAMB neurons changes pre-ecdysis duration. Bars represent mean time (± SEM, min) of the switch from pre-ecdysis to ecdysis onset relative to pre-ecdysis initiation (time zero). Data was analyzed using Mann-Whitney test (* p < 0.01; ** p < 0.001; *** p < 0.0001.) (B) ETHR over-expression in kinin neurons causes increased pre-ecdysis duration due to premature onset of pre-ecdysis. On the other hand, over-expression of ETHR in CAMB neurons accelerates the switch to ecdysis behavior due to increased sensitivity to ETH. See model below depicting how ETHR overexpression in kinin neurons or CAMB neurons affects pre-ecdysis duration. Error-bars represent standard error of mean (S.E.M). Data was analyzed using Mann-Whitney test (** P < 0.001, *** P < 0.0001).