Fig 2. Neutrophil yield using density gradient isolation or negative selection.

(A) Neutrophil yield (10⁶/mL) from whole blood isolated by Polymorphprep and negative selection (Beads). Data represents n = 5 paired neutrophil isolations (**p<0.01). (B) Neutrophil yield (10⁶/mL) from whole blood isolated by Ficoll-Paque (Ficoll) and negative selection (Beads). Data represent n = 5 experiments in which neutrophil enrichment from the Ficoll-Paque granulocyte pellet was carried out using negative selection (**p<0.01).