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(A) Immunoblot analysis of PTEN, phospho-Akt (S473), total Akt, and actin in ES, OS, RMS, breast cancer (MCF-7), and prostate cancer (LNCaP) cell lines. (B) Known PI3K and MAPK pathway mutations in cell lines evaluated in A. ES, Ewing sarcoma; ERMS, embryonal rhabdomyosarcoma; DEL, deletion; INS, insertion.
