Skip to main content
. 2015 Sep 24;10(9):e0133610. doi: 10.1371/journal.pone.0133610

Fig 5. Buparlisib inhibits anchorage independent growth and induces apoptosis.

Fig 5

(A) Plot of number and size of ES (TC-174, A4573), OS (KHOS, MNNG), and RMS (RD) colonies formed in soft agar in the presence of DMSO or increasing concentrations of buparlisib. Columns represent the average of three replicates, error bars represent standard deviation. (B) Representative pictures of TC-174 soft agar colonies. (C) Immunoblot analysis of cleaved PARP in ES (TC-174, TC-32, A4573), OS (KHOS), and RMS (RD, SJCRH30) treated with increasing doses of buparlisib for 24 hours. (D) Cell cycle analysis after 24 hours of buparlisib treatment of ES (TC-174, A4573), OS (KHOS), and RMS (RD) cell lines. Data are the mean of two independent experiments.