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. 2015 Jul 8;12(4):4837–4842. doi: 10.3892/mmr.2015.4058

Figure 4.

Figure 4

Overexpressed TET1 inhibits ACHN cell proliferation and metastasis, and improves apoptosis. (A) TET1 was overexpressed in the ACHN renal carcinoma cell line. The ACHN cells were transfected with pCMV6-XL5-TET1 and screened using puromycin to generate a stable cell line. The protein levels in the whole cell extracts were analyzed using western blot analysis. (B) Proliferation rate of the TET1-overexpressed ACHN cells was determined using flow cytometric analysis. The PI was calculated as follows: PI= (S + G2/M) / (S + G2/M + G0/G1). (C) Metastatic ability of the TET1-overexpressed ACHN cells was determined using a Transwell assay. (D) Apoptotic rate of the TET1 overexpressed ACHN cells was analyzed using flow cytometry subsequent to labeling with PI and annexin V. (*P<0.05, compared with the vector group). TET1, ten-eleven translocation methylcytosine dioxygenase 1; FITC, fluorescein isothiocyanate.