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. 2015 Aug 6;12(4):5619–5624. doi: 10.3892/mmr.2015.4189

Figure 1.

Figure 1

Lentiviral vectors for the NLK siRNA were constructed and demonstrated to be specific and potent for silencing NLK expression in Hep-2 cells. (A) Expression of GFP from uninfected Hep-2 cells (Con) and cells infected with the Lv-shNLK and Lv-shCon indicates the lentivirus infection efficiency in Hep-2 cells (magnification, ×10). (B) Quantitative polymerase chain reaction of NLK mRNA levels from uninfected Hep-2 cells (Con) and cells infected with the Lv-shNLK and Lv-shCon. (C) Western blot analysis of NLK protein expression in uninfected Hep-2 cells (Con) and cells infected with the Lv-shNLK and Lv-shCon. NLK, nemo-like kinase; GFP, green-fluorescent protein.