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. 2015 Jul 20;12(4):4981–4988. doi: 10.3892/mmr.2015.4099

Figure 3.

Figure 3

Determination of lentiviral transfection efficiency. (A–C) Fluorescence intensity of adipose-derived stem cells in the (A) Lenti-BDNF-GFP transfected group (magnification, ×200), (B) Lenti-NT-3-RFP transfected group (magnification, ×200) and the (C) Lenti-BDNF-GFP and Lenti-NT-3-RFP co-transfected group (magnification, ×100). Number of (D) GFP-positive cells in the Lenti-BDNF-GFP transfected group, (E) RFP-positive cells in the Lenti-NT-3-RFP transfected group and (F) GFP- and RFP-positive cells in the Lenti-BDNF-GFP and Lenti-NT-3-RFP co-transfected group, as determined by flow cytometry. RFP, red fluorescent protein; GFP, green fluorescent protein; BDNF, brain-derived neurotophic factor; NT-3, neurotrophin-3.