Skip to main content
. 2015 Jul 7;12(4):5086–5092. doi: 10.3892/mmr.2015.4049

Figure 1.

Figure 1

Girdin shRNA decreases the gene expression of girdin. (A and B) Protein expression levels of girdin in the U373, U251, A172, U87-MG and SHG-44 cell lines were detected using western blot, with β-actin as a reference. (C) Changes in mRNA levels of girdin were detected using reverse transcription-quantitative polymerase chain reaction, following transfection with girdin shRNA. The relative mRNA expression level in each sample was quantified using the 2−∆∆Ct method. (D and E) Expression levels of girdin were measured using western blot analysis following transfection. Each experiment was repeated three times. The experimental results are presented as the mean ± standard deviation. ***P<0.001, compared with the NC group. shRNA, short hairpin RNA; NC, negative control.